a selective inhibitor of Peptidyl Transferase Center (PTC): inhibits protein synthesis; emetic; enhances bronchial gland secretion and possesses relaxing effect on bronchial muscles; probably dopaminergic receptor agonist.

Phenantridine alkaloid C₁₆H₁₇NO₄.HCl from plants of the Amaryllidaceae family.

• Molecular Weight : 323.77 Da.
  
  
• Melting Point : 210-212 °C.
  
  
• Solubility in : slightly in 95% alcohol; not well in water; not in chloroform.
  
  
• Purity min. : 98 %. (IR, mass spectrometry, NMR, non aqueous titration)
  
  
• Physical Form : fine crystal powder.
  
  
• Biological Activity : one of the most active inhibitors of protein synthesis catalyzed by eucaryotic ribosomes among this group of Amaryllidaceae alkaloids. prevents the transfer of the N-acetylleucyl residue from 3'-terminal fragments of AcLeu-tRNA onto puromycin [3-4]. binds to 60S ribosomal subunit isolated from wheat germ with Ka = 8.65x102 M-1. Changes their conformation in the PTC region. These events can result in blocking the oligonucleotide model donor adsorption [1]. the emetic effect on dogs starts at the dose of 0.5 mg/kg s.c.
  
  
• Toxicity (LD₅₀) : mice (orally): 180 mg/kg mice (s.c.): 108 mg/kg
  
  
• Storage recommendations : Stable in dry state, store at +4°C, in dark place.Solution for use: 10^-4 - 10^-2 g/ml. For experiment in vivo: - as an emetic: 0.1 - 1.0 mg/kg s.c. - as a cholinesterase inhibitor: 5 - 10 mg/kg s.c.
  
  
• CAS Reg. No. : 2188-68-3
  
  
• Bibliography :
  
  1. Kukhanova M. et al., "Peptidyltransferase center of ribosomes. On the mechanism of action of alkaloid Lycorine." FEBS Letters (1983). 160(1,2):129-133
  2. Yunusov.S.Y., Alkaloids. FAN, Tashkent (1983) (Russian).
  3. Carrasco L. et al., FEBS Letters (1975). 52:236-239
  4. Jimenez A. et al., Biochim. Biophys. Acta (1976). 425:342-348