M₂ muscarinic receptor antagonist; myorelaxant; allows the production of 3-a[125I]Iodo-Imperialine by substitution of bromine by [125I] iodine.

Steroid alkaloid C₂₇H₄₂NO₂Br derivative of Imperialine from the plant Petilium eduardi, and P. raddeanae.

• Molecular Weight : 492 Da.
  
  
• Melting Point : 199-202°C.
  
  
• Solubility in : well in chloroform; slightly in methanol and ethanol; not well in acetone.
  
  
• Purity min. : 98 % (TLC).
  
  
• Biological Activity : Possesses M₂ selective activity. ED50 on cats under anaesthesia (i.v.) 0.015 mg/kg : 65% of Atropine activity on heart, 1.7% on isolated ileum, 0.16% on salivary gland. Anti-depolarising myorelaxant. At sublethal doses: muscular weakness, irregular and slow respiration, exophthalm, depression.Effects diminished after 10-15 min, recovery after 60 min.
  
  
• Toxicity (LD₅₀) : mice (i.v.): 48 mg/kg.
  
  
• Storage recommendations : Store at +4°C, in dark place.
  
  
• CAS Reg. No. : 176039-27-3
  
  
• Merck Index 11th Ed. : 4840
  
  
• Bibliography :
  
  1. Eglen et al., "Characterisation of the interaction of the cervane alkaloid, imperialine, at Muscarinic receptors in vitro." Naunyn-Schmiedeberg’s Arch. Pharmacol. (1992) 346:144-152
  2. on 3-b-Bromo-Imperialine: Mirzaev, unbublished results.