Excitatory anti-insect selective scorpion b-toxin.

Polypeptide, of 77 amino acids cross-linked by 4 disulfide bridges. Recombinant toxin produced in Eschirichia coli initialy from Buthotus judaicus scorpion venom.

• Molecular Weight : 8,575 Da.
  
  
• Solubility in : in water and aqueous solutions.
  
  
• Purity min. : 98%.
  
  
• Biological Activity : Toxic exclusively to insects. Effective dose 50% in blowfly larvae (ED50) = 14 ng/100 mg body weight. Equilibrium dissociation constant, Kd, determined in binding assays to cockroach neuronal membranes = 0.15 nM (ref. 3).
  
  
• Toxicity (LD₅₀) : no mammal toxicity.
  
  
• Storage recommendations : Store at 4°C.
  
  
• Bibliography :
  
  1. Froy, O., Zilberberg, N., Gordon, D., Turkov, M., Gilles, N., Stankiewicz, M., Pelhate, M., Loret, E., Oren, D., Shaanan, B. and Gurevitz, M. (1999) The putative bioactive surface of insect-selective scorpion excitatory neurotoxins. J.Biol.Chem. 274, 5769-5776.
  2. Oren, D. A., Froy, O., Amit, E., Kleinberger-Doron, N., Gurevitz, M. and Shaanan, B. (1998) An excitatory scorpion toxin with a distinctive feature: an additional a-helix at the C-terminus and its implications for interaction with insect sodium channels. Structure 6, 1095-1103
  3. Cohen, L., Karbat, I., Gilles, N., Froy, O., Angelovici, R., Gordon, D., Gurevitz, M.(2004) Dissect. of the functional surface of an anti-insect excit. toxin illuminates a putative 'hot spot' common to all scorpion beta-toxins affecting Na channels, J.Biol.Chem., 279, 8206-8211