Maurocalcin (MCa) - LATOXAN Technical Data Sheet
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L8225 Maurocalcin (MCa)

An activator of Ca2+ release channel/ryanodine receptors.

33-mer basic peptide cross-linked by three disulphide bridges. Synthetic, from Scorpio maurus palmatus scorpion venom.


PRICES
QuantityUS DollarsEURO
25 µg 280.00 200.00
5 x 25 µg1 120.00 800.00


  • Molecular Weight : 3857.2 Da

  • Solubility in : aqueous solution.

  • Purity min. : >99%

  • Physical Form : white powder

  • Biological Activity : Agonist of ryanodine receptor type 1, 2 and 3 - Potently and reversibly modifies channel gating behaviour of the type 1 ryanodine receptor (RyR1) by inducing increased opening probabilities and a prominent subconductance behaviour.

    Binds to a different site than ryanodine.
    Class of biological activity: Ca2+ channel activator.
    At cellular level: Myotubes, HEK293 cells expressing RyR1.
    Potency: EC50 at 12 to 20 nM.
    Field of use: Therapeutic potential, notably as candidate immuno-suppressive drugs, and for the treatment of diseases induced by dysfunction of calcium channels, in man.
    Research in pharmacology and physiology.

  • Toxicity (LD50) : Lethal dose: 20 µg/mice (ICV).
    Route of administration: Intracerebroventricular & intravenous.

  • Storage recommendations : Stable at room temperature, store preferably at -20°C.

  • Reconstitution : in aqueous solution.

  • Safety recommendations : Use gloves, wear mask, manipulate under hood.

  • Bibliography :
    1. Fajloun Z, Kharrat R, Chen L, Lecomte C, di Luccio E, Bichet D, El Ayeb M, Rochat H, Allen PD, Pessah IN, De Waard, M & Sabatier J-M.Chemical synthesis and characterization of maurocalcine, a scorpion toxin that activates Ca2+ release channel / ryanodine receptors. FEBS Letters 469: 179-185 (2000).
    2. Chen L, Estève E, Sabatier JM, Ronjat M, De Waard M, Allen PD & Pessah IN.Maurocalcine and peptide A stabilize distinct subconductance states of ryanodine receptor type 1 (RyR1) revealing a proportional gating mechanism. Journal of Biological Chemistry 278: 16095-16106 (2003).
    3. Estève E, Smida-Rezgui S, Sarkozi S, Szegedi C, Regaya I, Chen L, Altafaj X, Rochat H, Allen P, Pessah IN, Marty I, Sabatier JM, Jona I, De Waard M & Ronjat M.Critical amino acid residues determine the binding affinity and the Ca2+ release efficacy of maurocalcine in skeletal muscle cells. Journal of Biological Chemistry 278: 37822-37831 (2003).
    4. Estève E, Mabrouk K, Dupuis A, Smida-Rezgui S, Altafaj X, Grunwald D, Platel JC, Andreotti N, Marty I, Sabatier JM, Ronjat M & De Waard M.Transduction of the scorpion toxin maurocalcine into cells – Evidence that the toxin crosses the plasma membrane. Journal of Biological Chemistry 280: 12833-12839 (2003).
    5. Szappanos H, Smida-Rezgui S, Cseri J, Simut C, Sabatier JM, De Waard M, Kovács L, Csernoch L & Ronjat M.Differential effects of Maurocalcine on Ca2+ release events and depolarisation induced Ca2+ release in rat skeletal muscle. Journal of Physiology 565: 843-853 (2005).
    6. Boisseau S, Mabrouk K, Garmy N, Collin V, Tadmouri A, Mikati A, Sabatier JM, Ronjat M, Fantini J, De Waard M.Cell penetration properties of maurocalcine, a natural venom peptide active on the intracellular ryanodine receptor. Biochimica Biophysica Acta Biomembranes 1758 : 308-319 (2005).



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    Revised : 16/01/2008 10:05 - Copyright © Latoxan