(Syn. : HwTx-IV , mu-theraphotoxin-Hs2a, mu-TRTX-Hs2a)
HUWENTOXIN-IV | Product ID | L8434-100UG |
| Name | HUWENTOXIN-IV |
| Molecular formula | C174H277N51O52S6 |
| Description |
A 35 amino-acids peptide with 3 disulfide bridges , synthetic, initially isolated from the venom of the Chinese gold earth tiger spider Ornithoctonus huwena, also known as Cyriopagopus schmidti, Haplopelma schmidti, Haplopelma huwenum. |
| Biological activity | Huwentoxin IV(HwTx-IV) acts selectively on Tetrodotoxin-sensitive (TTX-S) voltage-gated sodium channels, with an IC50 of 30 nM in rat DRG neurons. It preferentially inhibits neuronal voltage-gated sodium channel subtype hNav1.7 (SCN9A, IC50 is 26 nM), rNav1.2 (SCN2A, IC50 is 150 nM), and rNav1.3 (SCN3A, IC50 is 338 nM). Huwentoxin IV inhibits the activation of sodium channels by trapping the voltage sensor of domain II of the site 4 in the inward, closed configuration. Shows a weak or no inhibition on rNav1.4/SCN4A (IC50=3.9 µM) and hNav1.5/SCN5A sodium channels (IC50=25->10 µM) (PubMed:18628201, PubMed:25658507). This toxin has also been shown to weakly inhibit Kv11.1/KCNH2/ERG1, Kv1.2/KCNA2 and Kv1.3/KCNA3. |
Packaging and Prices
| Packaging | Price (€) | Quantité | Buy Now |
|---|---|---|---|
| 1 Unité(s) | 90.00 | ||
| 5 Unité(s) | 360.00 | ||
| For quotation of big quantities, please Contact us | |||
Product Technical Documents
 | Safety Data Sheet Available on request, please Contact us Ref. SDS_L8434-100UG : unit price : free of charge |
Chemical and Physical Properties
| Product code | L8434-100UG | Molecular weight | 4107.20 Da |
| Product name | HUWENTOXIN-IV | Physical form | White lyophilized solid |
| CAS No. | 526224-73-7 | Melting point | |
| RTECS | NA | Solubility | water and saline buffer |
| UNIPROT | P83303 | Optical Activity | |
| PubChem SID | |||
| PubChem CID | 90488968 | ||
| Purity | > 98 % | ||
| Toxicity(LD50) | No Data | ||
| Chemical Name | |||
| Storage & Reconstitution Recommandations |
Stable at room temperature, store preferably at 4°C |
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| Savety Recommandations | |||
Bibliographic References
| Product code | L8434-100UG |
| Product name | HUWENTOXIN-IV |
- 1. Minassian NA., et al. (2013) Analysis of the structural and molecular basis of voltage-sensitive sodium channel inhibition by the spider toxin, Huwentoxin-IV (?-TRTX-Hh2a). JBC. ;
2. Peigneur S, et al. (2012) A natural point mutation changes both target selectivity and mechanism of action of sea anemone toxins. FASEB J. ;
3. Peng, K., et al. (2002) Function and solution structure of huwentoxin-IV, a potent neuronal tetrodotoxin (TTX)-sensitive sodium channel antagonist from Chinese bird spider Selenocosmia huwena, J Biol Chem. ;
4. Wang RL, et al. (2010) Mechanism of action of two insect toxins huwentoxin-III and hainantoxin-VI on voltage-gated sodium channels. ;
5. Xiao, Y., et al. (2008) Tarantula huwentoxin-IV inhibits neuronal sodium channels by binding to receptor site 4 and trapping the domain ii voltage sensor in the closed configuration, JBC. ;
6. Xiao, Y., et al. (2010) The tarantula toxins ProTx-II and huwentoxin-IV differentially interact with human Nav1.7 voltage sensors to inhibit channel activation and inactivation, Mol Pharmacol. ;
7. Xiao, Y., et al. (2011) Common molecular determinants of tarantula huwentoxin-IV inhibition of Na+ channel voltage sensors in domains II and IV, JBC.
Links
| Product code | L8434-100UG |
| Product name | HUWENTOXIN-IV |
| External Links |
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